Treatment was continued until at least two follicles of at least 18 mm had developed. Ovulation triggering was induced by 10000 IU human chorionic gonadotropin hormone (Pregnyl https://www.pandora-jewelry-uk.com/, Merck Sharp Dohme), and cumulus oocyte complexes were recovered by transvaginal ultrasound guided retrieval 36 hours thereafter.We adhered to an elective single embryo transfer policy: if one good quality embryo was available, we transferred one embryo.25 If more than one good quality embryo was available, suitable surplus embryos were cryopreserved. If no good quality embryos were available, two embryos would be transferred.

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